Description
Intended Uses: This human coagulation Factor XII antigen assay is intended for the quantitative determination of total Factor XII antigen in human plasma. For research use only.
Principle of the Assay: Human Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, anti-human Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody, which is proportional to the total Factor XII present in the samples, is reacted with the secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor XII. Color development is proportional to the concentration of Factor XII in the samples.
Background: Factor XII (aka Hageman Factor) is a single-chain, 615 amino acid glycoprotein zymogen [1]. Factor XII is activated by kallikrein [2]. Factor XIIa converts prekallikrein to kallikrein during the intrinsic pathway of the coagulation cascade. Although Factor XII is not thought to play an essential role in normal hemostasis, lack of Factor XII in a mouse model resulted in a 'severe defect' in thrombus formation [3]